Molecular Genetics Core

Michigan Diabetes Research Center (MDRC)

The Molecular Genetics Core provides services related to the application of genetic methodologies to diabetes-related research.

Access Our Services

All customers, request services by emailing dpolson@umich.edu.

Generation of targeted genetic rodent models, using CRISPR/Cas9 system in mouse or rat embryos:

Gene edited alleles (base changes/deletions)
Knockout alleles
loxP-flanked alleles
Large DNA fragment insertions targeting transgenes such as Cre and Flp recombinases or fluorescent reporters (e.g., GFP) to genes of interest
Cost includes targeting design, testing of guide RNAs for DNA targeting, and screening of potential founders by PCR and sequence analysis. Note: cost does NOT include injection costs
Application of CRISPR-Cas9 technology in specific genetic backgrounds is available on request, at extra cost

Consultation regarding design, generation, and production of specialty viral vectors:

Constitutive and inducible AAV viruses
Defective pseudotyped rabies viruses for tract-tracing, including AAV helper viruses
Under development: AAV-driven CRISPR/Cas9 reagents for gene ablation in animals; yellow fever virus for anterograde neuronal circuit tracing

David P. Olson, MD, PhD
Core Director
dpolson@umich.edu

Qing Zhu, MD
Research Lab Specialist Associate
qinzhu@umich.edu

Molecular Genetics Core
North Campus Research Complex (NCRC), Building 25-3753
dpolson@umich.edu